Biosensor Development for Immune Checkpoint Molecules

Biosensor Development for Immune Checkpoint Molecules 
Immune checkpoint molecules are critical in regulating immune responses and significantly affect disease diagnostics and therapeutic development. This project aims to develop and optimize a plasmonic biosensor for the sensitive and selective detection of immune checkpoint molecules, leveraging real-time, label-free analysis for high specificity and sensitivity. The primary objectives include optimizing the immobilization of capture molecules on plasmonic sensors through advanced surface functionalization techniques, characterizing molecular interactions to determine binding interactions, establishing robust regeneration protocols for sensor reusability, and validating performance using spiked biological samples. Optimization of buffer conditions will ensure effective regeneration while maintaining sensor performance. The expected outcome is a highly sensitive and reusable plasmonic chip-based immune checkpoint sensor, contributing to advancements in immunotherapy research, diagnostics, and biomarker discovery.