Increasing iron-binding capacity of bacteria

2016-2017 Summer
Faculty Department of Project Supervisor: 
Faculty of Engineering and Natural Sciences
Number of Students: 

Iron is a significant element for mostly all the organisms. It is used in several biological processes such as cell respiration, photosynthesis, DNA synthesis, gene regulation and tricarboxylic acid cycle, etc. Normally, bacteria can bind iron however, some bacteria have special proteins that bind the iron which increases the iron binding capacity. We took one of this genes which is responsible for synthesizing a protein called Ferric Binding Protein (FBP) from H. Influenzae and add this gene with a histidine residue at the end of the polypeptide chain to E. Coli bacteria with recombinant DNA method. Adding the FBP gene to the E. Coli makes the bacteria capable to synthesize the FBP.