Evaluation of different Cellulose Binding Modules (CBM) of various cellulosome complexes as an immobilization tag for paper-based biosensors.

Cellulosomes are multi-enzymatic complexes that are utilized by anaerobes to decompose plant based cellulosic structures [1] . The Cellulose Binding Module (CBM) is a small part of the cellulosome complex tasked with firm attachment to the cellulosic material [1] [2] . This specific attachment can be utilized in paper-based biosensors to create a simple, easy to use immobilization method [2] . In this project, different CBM structures will be evaluated using computational methods. A library of CBMs will be created. In the future, this library will be used to create CBMs with tags and CBMs fused with other enzymes or target molecules to create chimeric proteins that could self-attach to cellulosic materials.

References
[1] Bule, P., Alves, V. D., Israeli-Ruimy, V., Carvalho, A. L., Ferreira, L. M., Smith, S. P., Gilbert, H. J.,
Najmudin, S., Bayer, E. A., & Fontes, C. M. (2017). Assembly of ruminococcus flavefaciens
cellulosome revealed by structures of two cohesin-dockerin complexes. Scientific Reports, 7(1).
https://doi.org/10.1038/s41598-017-00919-w
[2] Vanderstraeten, J., Fonseca, M. J., De Groote, P., Grimon, D., Gerstmans, H., Kahn, A., Moraïs, S.,
Bayer, E. A., & Briers, Y. (2021). Combinatorial Assembly and optimisation of designer cellulosomes -
A galactomannan case study. https://doi.org/10.21203/rs.3.rs-1199416/v1